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Name:
Wright Giemsa Stain 1-Step
Synonym:
None
CAS:
0-00-0
Section 1 - Chemical Product
MSDS Name:Wright Giemsa Stain 1-Step
Synonym:None
Section 2 - COMPOSITION, INFORMATION ON INGREDIENTS
CAS# Chemical Name emsds EINECS#
0-00-0 Glycerine 8.3 200-289-5
Hazard Symbols: XN C
Risk Phrases: 10 22 34

Section 3 - HAZARDS IDENTIFICATION
EMERGENCY OVERVIEW
Flammable. Harmful if swallowed. Causes burns.Corrosive.
Potential Health Effects
Eye:
Causes eye burns. Vapors may cause eye irritation. May cause painful sensitization to light.
Skin:
Causes skin burns. May be absorbed through the skin in harmful amounts. Maleic acid passes through intact skin.
Ingestion:
May be fatal or cause blindness if swallowed. May cause gastrointestinal irritation with nausea, vomiting and diarrhea. May cause systemic toxicity with acidosis. May cause liver and kidney damage. May cause central nervous system depression, characterized by excitement, followed by headache, dizziness, drowsiness, and nausea. Advanced stages may cause collapse, unconsciousness, coma and possible death due to respiratory failure.
Inhalation:
Harmful if inhaled. May cause respiratory tract irritation. May cause liver and kidney damage. May cause visual impairment and possible permanent blindness. May cause effects similar to those described for ingestion. May cause narcotic effects in high concentration. May cause drowsiness, unconsciousness, and central nervous system depression.
Chronic:
Prolonged or repeated skin contact may cause dermatitis. Chronic inhalation and ingestion may cause effects similar to those of acute inhalation and ingestion.

Section 4 - FIRST AID MEASURES
Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid immediately. Do NOT allow victim to rub eyes or keep eyes closed.
Skin:
Get medical aid immediately. Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Discard contaminated clothing in a manner which limits further exposure.
Ingestion:
If victim is conscious and alert, give 2-4 cupfuls of milk or water.
Never give anything by mouth to an unconscious person. Get medical aid immediately.
Inhalation:
Get medical aid immediately. Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen.
Notes to Physician:

Section 5 - FIRE FIGHTING MEASURES
General Information:
Containers can build up pressure if exposed to heat and/or fire. As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. Vapors may form an explosive mixture with air.
Vapors can travel to a source of ignition and flash back. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Use water spray to keep fire-exposed containers cool. Flammable liquid and vapor. Vapors may be heavier than air. They can spread along the ground and collect in low or confined areas. May be ignited by heat, sparks, and flame. Containers may explode when heated.
Extinguishing Media:
For small fires, use dry chemical, carbon dioxide, water spray or alcohol-resistant foam. Use water spray to cool fire-exposed containers. Water may be ineffective. Do NOT use straight streams of water. For large fires, use dry chemical, carbon dioxide, alcohol-resistant foam, or water spray. Cool containers with flooding quantities of water until well after fire is out.

Section 6 - ACCIDENTAL RELEASE MEASURES
General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks:
Remove all sources of ignition. Absorb spill using an absorbent, non-combustible material such as earth, sand, or vermiculite. Do not use combustible materials such as sawdust. Provide ventilation. A vapor suppressing foam may be used to reduce vapors. Water spray may reduce vapor but may not prevent ignition in closed spaces.

Section 7 - HANDLING and STORAGE
Handling:
Wash thoroughly after handling. Ground and bond containers when transferring material. Do not get in eyes, on skin, or on clothing.
Empty containers retain product residue, (liquid and/or vapor), and can be dangerous. Keep container tightly closed. Keep away from heat, sparks and flame. Do not ingest or inhale. Use only in a chemical fume hood. Do not pressurize, cut, weld, braze, solder, drill, grind, or expose empty containers to heat, sparks or open flames.
Storage:
Keep away from heat, sparks, and flame. Keep away from sources of ignition. Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances.

Section 8 - EXPOSURE CONTROLS, PERSONAL PROTECTION
Engineering Controls:
Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate general or local exhaust ventilation to keep airborne concentrations below the permissible exposure limits. Use only under a chemical fume hood.
Exposure Limits CAS# 0-00-0: CAS# 56-81-5: United Kingdom, WEL - TWA: 10 mg/m3 TWA (mist) United Kingdom, WEL - STEL: 30 mg/m3 STEL (mist) United States OSHA: 15 mg/m3 TWA (total dust); 5 mg/m3 TWA (respirable fraction) Belgium - TWA: 10 mg/m3 VLE (mist) France - VME: 10 mg/m3 VME Malaysia: 10 mg/m3 TWA (mist) Netherlands: 10 mg/m3 MAC (mist) Spain: 10 mg/m3 VLA-ED CAS# 67-56-1: United Kingdom, WEL - TWA: 200 ppm TWA; 266 mg/m3 TWA United Kingdom, WEL - STEL: 250 ppm STEL; 333 mg/m3 STEL United States OSHA: 200 ppm TWA; 260 mg/m3 TWA Belgium - TWA: 200 ppm VLE; 266 mg/m3 VLE Belgium - STEL: 250 ppm VLE; 333 mg/m3 VLE France - VME: 200 ppm VME; 260 mg/m3 VME France - VLE: 1000 ppm VLE; 1300 mg/m3 VLE Germany: 200 ppm TWA; 270 mg/m3 TWA Germany: Skin absorber Japan: 200 ppm OEL; 260 mg/m3 OEL Malaysia: 200 ppm TWA; 262 mg/m3 TWA Netherlands: 200 ppm MAC; 260 mg/m3 MAC Russia: 5 mg/m3 TWA Spain: 200 ppm VLA-ED; 266 mg/m3 VLA-ED Spain: 250 ppm VLA-EC; 333 mg/m3 VLA-EC CAS# 110-16-7: CAS# 124-40-3: United Kingdom, WEL - TWA: 2 ppm TWA; 3.8 mg/m3 TWA United Kingdom, WEL - STEL: 6 ppm STEL; 11 mg/m3 STEL United States OSHA: 10 ppm TWA; 18 mg/m3 TWA Belgium - TWA: 2 ppm VLE; 3.8 mg/m3 VLE Belgium - STEL: 5 ppm VLE; 9.4 mg/m3 VLE France - VLE: 10 ppm VLE; 18 mg/m3 VLE Germany: 2 ppm TWA; 3.7 mg/m3 TWA Japan: 10 ppm OEL; 18 mg/m3 OEL Malaysia: 5 ppm TWA; 9.2 mg/m3 TWA Netherlands: 1 ppm MAC; 1.8 mg/m3 MAC Russia: 1 mg/m3 TWA Spain: 2 ppm VLA-ED; 3.8 mg/m3 VLA-ED Spain: 5 ppm VLA-EC; 9.4 mg/m3 VLA-EC CAS# 506-59-2: CAS# 531-53-3: CAS# 660-68-4: CAS# 1310-58-3: United Kingdom, WEL - STEL: 2 mg/m3 STEL Belgium - STEL: 2 mg/m3 VLE France - VLE: 2 mg/m3 VLE Japan: 2 mg/m3 Ceiling Malaysia: 2 mg/m3 Ceiling Spain: 2 mg/m3 VLA-EC CAS# 7447-40-7: Russia: 5 mg/m3 TWA CAS# 9002-93-1: CAS# 10237-70-4: CAS# 68988-92-1: Personal Protective Equipment Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHAs eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin:
Wear appropriate protective gloves to prevent skin exposure.
Clothing:
Wear appropriate protective clothing to prevent skin exposure.
Respirators:
Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced.

Section 9 - PHYSICAL AND CHEMICAL PROPERTIES

Physical State: Liquid
Color: dark blue
Odor: Alcohol
pH: Not available.
Vapor Pressure: 96 mm Hg
Viscosity: Not available.
Boiling Point: 66 deg C
Freezing/Melting Point: -98 deg C
Autoignition Temperature: Not available.
Flash Point: 54 deg F ( 12.22 deg C)
Explosion Limits, lower: 6.0
Explosion Limits, upper: 36.0
Decomposition Temperature:
Solubility in water: Infinite
Specific Gravity/Density:
Molecular Formula:
Molecular Weight:

Section 10 - STABILITY AND REACTIVITY
Chemical Stability:
Stable under normal temperatures and pressures.
Conditions to Avoid:
Incompatible materials, ignition sources, excess heat.
Incompatibilities with Other Materials:
The incompatibilties associated with potassium hydroxide include: acids, bromoform, chlorine dioxide, cyclopentadiene, germanium, hyponitrous acid, maleic anhydride, nitroalkanes, nitrobenzene, nitrogen trichloride, 2-nitrophenol, potassium peroxodisulfate, sugars, 2,2,3,3-tetrafluoropropanol, tetrahydrofuran, thorium dicarbide, 2,4,6trinitrotoluene, acetic acid, acrolein, acrylonitrile, chlorine+hydrogen peroxide, chloroform+methanol, phosphorus, trichloroethylene, tetrachlorethane. It reacts violently with o-nitrophenol. Reactions with 1,2-dichloroethylene produce chloroacetylene which is explosive and spontaneously flammable in air. Potassium hydroxide generates large amounts of heat when in contact with water and may steam and splatter. Potassium hydroxide is corrosive to metals such as aluminum, tin, and zinc to cause formation of flammable hydrogen gas. The incompatibilities associated with glycerol include: acetic anhydride, calcium hypochlorite, chromium oxide, fluorine+lead oxide, perchloric acid+lead oxide, and potassium permanganate. Wrights stain is incompatible with strong oxidizing agents. The incompatibilities associated with methanol include: oxidants, phosphorus (III) oxide, dichloromethane, acetyl bromide, active metals, alkyl aluminum salts, beryllium dihydride, carbon tetrachloride+metals, chloroform+heat, chloroform+sodium hydroxide, cyanuric chloride, diethyl zinc, potassium tertbutoxide, chromic anhydride, lead perchlorate, perchloric acid, and chloroform+potassium hydroxide. Maleic acid is incompatible with oxidizing agents, strong bases, and alkali metals.
Hazardous Decomposition Products:
Carbon monoxide, oxides of nitrogen, carbon dioxide, oxides of carbon.
Hazardous Polymerization: Will not occur.

Section 11 - TOXICOLOGICAL INFORMATION
RTECS#:
CAS# 0-00-0 unlisted.
CAS# 56-81-5: MA8050000 CAS# 67-56-1: PC1400000 CAS# 110-16-7: OM9625000 CAS# 124-40-3: IP8750000 CAS# 506-59-2: IQ0220000 CAS# 531-53-3: SP5660000 CAS# 660-68-4: IA3084000 CAS# 1310-58-3: TT2100000 CAS# 7447-40-7: TS8050000 CAS# 9002-93-1: MD0907700 YM0616666 YM0683332 CAS# 10237-70-4 unlisted.
CAS# 68988-92-1 unlisted.
LD50/LC50:
Not available.
CAS# 56-81-5: Draize test, rabbit, eye: 126 mg Mild; Draize test, rabbit, eye: 500 mg/24H Mild; Draize test, rabbit, skin: 500 mg/24H Mild; Inhalation, rat: LC50 = >570 mg/m3/1H; Oral, mouse: LD50 = 4090 mg/kg; Oral, rabbit: LD50 = 27 gm/kg; Oral, rat: LD50 = 12600 mg/kg; Skin, rabbit: LD50 = >10 gm/kg.
CAS# 67-56-1: Draize test, rabbit, eye: 40 mg Moderate; Draize test, rabbit, eye: 100 mg/24H Moderate; Draize test, rabbit, skin: 20 mg/24H Moderate; Inhalation, rabbit: LC50 = 81000 mg/m3/14H; Inhalation, rat: LC50 = 64000 ppm/4H; Oral, mouse: LD50 = 7300 mg/kg; Oral, rabbit: LD50 = 14200 mg/kg; Oral, rat: LD50 = 5600 mg/kg; Skin, rabbit: LD50 = 15800 mg/kg.
CAS# 110-16-7: Draize test, rabbit, eye: 100 mg Severe; Draize test, rabbit, eye: 1%/2M Severe; Draize test, rabbit, skin: 500 mg/24H Mild; Inhalation, rat: LC50 = >720 mg/m3/1H; Oral, mouse: LD50 = 2400 mg/kg; Oral, rat: LD50 = 708 mg/kg; Skin, rabbit: LD50 = 1560 mg/kg.
CAS# 124-40-3: Draize test, rabbit, eye: 50 mg/5M; Inhalation, mouse: LC50 = 4725 ppm/2H; Inhalation, rat: LC50 = 4540 ppm/6H; Oral, mouse: LD50 = 316 mg/kg; Oral, rabbit: LD50 = 240 mg/kg; Oral, rat: LD50 = 698 mg/kg.
CAS# 506-59-2: Oral, mouse: LD50 = 8100 mg/kg; Oral, rabbit: LD50 = 1600 mg/kg; Oral, rat: LD50 = 1070 mg/kg.
CAS# 531-53-3.
CAS# 660-68-4: Oral, mouse: LD50 = 4860 mg/kg; Oral, rat: LD50 = 9900 mg/kg.
CAS# 1310-58-3: Draize test, rabbit, skin: 50 mg/24H Severe; Oral, rat: LD50 = 273 mg/kg.
CAS# 7447-40-7: Draize test, rabbit, eye: 500 mg/24H Mild; Oral, mouse: LD50 = 1500 mg/kg; Oral, rat: LD50 = 2600 mg/kg.
CAS# 9002-93-1: Draize test, rabbit, eye: 10 uL/24H Moderate; Draize test, rabbit, skin: 500 uL/24H Mild; Oral, rat: LD50 = 1800 mg/kg; Oral, rat: LD50 = 3800 mg/kg; Oral, rat: LD50 = 1900 mg/kg.
CAS# 10237-70-4.
CAS# 68988-92-1.
Carcinogenicity:
Tris-Hydroxymethyl Amino Methane - Not listed by ACGIH, IARC, or NTP.
Glycerine - Not listed by ACGIH, IARC, or NTP.
Methanol - Not listed by ACGIH, IARC, or NTP.
Maleic acid - Not listed by ACGIH, IARC, or NTP.
Dimethylamine - Not listed by ACGIH, IARC, or NTP.
Dimethylamine hydrochloride - Not listed by ACGIH, IARC, or NTP.
Giemsa Stain Azure A - Not listed by ACGIH, IARC, or NTP.
Diethylamine hydrochloride - Not listed by ACGIH, IARC, or NTP.
Potassium hydroxide - Not listed by ACGIH, IARC, or NTP.
Potassium chloride - Not listed by ACGIH, IARC, or NTP.
Ethoxylated p-tert-octylphenol - Not listed by ACGIH, IARC, or NTP.
2-butenedioic acid (z)-, potassium salt - Not listed by ACGIH, IARC, or NTP.
Wright Stain - Not listed by ACGIH, IARC, or NTP.
Other:
See actual entry in RTECS for complete information.

Section 12 - ECOLOGICAL INFORMATION

Section 13 - DISPOSAL CONSIDERATIONS
Dispose of in a manner consistent with federal, state, and local regulations.

Section 14 - TRANSPORT INFORMATION

IATA
Shipping Name: Not regulated.
Hazard Class:
UN Number:
Packing Group:
IMO
Shipping Name: Not regulated.
Hazard Class:
UN Number:
Packing Group:
RID/ADR
Shipping Name: Not regulated.
Hazard Class:
UN Number:
Packing group:
USA RQ: CAS# 67-56-1: 5000 lb final RQ; 2270 kg final RQ
USA RQ: CAS# 110-16-7: 5000 lb final RQ; 2270 kg final RQ
USA RQ: CAS# 124-40-3: 1000 lb final RQ; 454 kg final RQ
USA RQ: CAS# 1310-58-3: 1000 lb final RQ; 454 kg final RQ

Section 15 - REGULATORY INFORMATION

European/International Regulations
European Labeling in Accordance with EC Directives
Hazard Symbols: XN C
Risk Phrases:
R 10 Flammable.
R 22 Harmful if swallowed.
R 34 Causes burns.
Safety Phrases:
S 16 Keep away from sources of ignition - No
smoking.
S 24/25 Avoid contact with skin and eyes.
S 28B After contact with skin, wash immediately with
plenty of water and soap.
S 45 In case of accident or if you feel unwell, seek
medical advice immediately (show the label where
possible).
WGK (Water Danger/Protection)
CAS# 0-00-0: No information available.
CAS# 56-81-5: 0
CAS# 67-56-1: 1
CAS# 110-16-7: 1
CAS# 124-40-3: 2
CAS# 506-59-2: 1
CAS# 531-53-3: No information available.
CAS# 660-68-4: 1
CAS# 1310-58-3: 1
CAS# 7447-40-7: 1
CAS# 9002-93-1: 1
CAS# 10237-70-4: 1
CAS# 68988-92-1: No information available.
Canada
CAS# 56-81-5 is listed on Canadas DSL List.
CAS# 67-56-1 is listed on Canadas DSL List.
CAS# 110-16-7 is listed on Canadas DSL List.
CAS# 124-40-3 is listed on Canadas DSL List.
CAS# 506-59-2 is listed on Canadas DSL List.
CAS# 531-53-3 is listed on Canadas DSL List.
CAS# 660-68-4 is listed on Canadas DSL List.
CAS# 1310-58-3 is listed on Canadas DSL List.
CAS# 7447-40-7 is listed on Canadas DSL List.
CAS# 9002-93-1 is listed on Canadas DSL List.
CAS# 10237-70-4 is listed on Canadas DSL List.
CAS# 68988-92-1 is listed on Canadas DSL List.
CAS# 0-00-0 is not listed on Canadas Ingredient Disclosure List.
CAS# 56-81-5 is not listed on Canadas Ingredient Disclosure List.
CAS# 67-56-1 is listed on Canadas Ingredient Disclosure List.
CAS# 110-16-7 is listed on Canadas Ingredient Disclosure List.
CAS# 124-40-3 is listed on Canadas Ingredient Disclosure List.
CAS# 506-59-2 is not listed on Canadas Ingredient Disclosure List.
CAS# 531-53-3 is not listed on Canadas Ingredient Disclosure List.
CAS# 660-68-4 is not listed on Canadas Ingredient Disclosure List.
CAS# 1310-58-3 is listed on Canadas Ingredient Disclosure List.
CAS# 7447-40-7 is not listed on Canadas Ingredient Disclosure List.
CAS# 9002-93-1 is listed on Canadas Ingredient Disclosure List.
CAS# 10237-70-4 is not listed on Canadas Ingredient Disclosure List.
CAS# 68988-92-1 is not listed on Canadas Ingredient Disclosure List.
US FEDERAL
TSCA
CAS# 0-00-0 is not listed on the TSCA inventory.
It is for research and development use only.
CAS# 56-81-5 is listed on the TSCA inventory.
CAS# 67-56-1 is listed on the TSCA inventory.
CAS# 110-16-7 is listed on the TSCA inventory.
CAS# 124-40-3 is listed on the TSCA inventory.
CAS# 506-59-2 is listed on the TSCA inventory.
CAS# 531-53-3 is listed on the TSCA inventory.
CAS# 660-68-4 is listed on the TSCA inventory.
CAS# 1310-58-3 is listed on the TSCA inventory.
CAS# 7447-40-7 is listed on the TSCA inventory.
CAS# 9002-93-1 is listed on the TSCA inventory.
CAS# 10237-70-4 is not listed on the TSCA inventory.
It is for research and development use only.
CAS# 68988-92-1 is listed on the TSCA inventory.